Figure 1
From: Definition of a Skp2-c-Myc Pathway to Expand Human Beta-cells
Proliferative response and p27kip1Cip/Kip expression in T2D human β-cells.
(A) Dispersed human islets from normal (Normal) or T2D (Type 2) donors were transduced with control adenovirus (“CTL”) or with Ad.cdk6 and Ad.cyclin D3 (“C6 + D3”). Immunolabelling for Ki67 is shown in red, insulin in green and DAPI in blue. (B) Scattered plot of % of Ki67+/insulin + cells in response to cdk6 and cyclin D3 in non-diabetic β-cells (NL) (n = 8) and in β-cells from T2D donors (T2D) (n = 5). (C) Quantitation of % of Ki67+/insulin + cells. Bars indicate mean ± SEM. (D) Dispersed human islets from normal (Normal) (n = 7) or T2D (Type 2) (n = 6) donors were transduced with control adenovirus (“CTL”) or with Ad.cdk6 and Ad.cyclin D3 (“C6 + D3”). Immunolabelling for p27kip1cip/kip is shown in red and insulin in green. (E) Quantification of data in C at 72 hours after cdk6 + cyclin D3 transduction. Bars indicate mean ± SEM of the % nuclear localization of the p27kip1 shown in insulin+ (beta) cells.
