Figure 2
From: Definition of a Skp2-c-Myc Pathway to Expand Human Beta-cells
Effect of p27kip1 overexpression on human β-cell proliferation.
(A) Dispersed human islets from normal donors (n = 5) were transduced with control adenovirus (CTL) or with of Ad.p27kip1. Immunolabelling for p27kip1 is shown in red, insulin in green and DAPI in blue. (B) Quantitation of the % nuclear localization of the p27kip1 shown in insulin+ (beta) cells with increasing doses of adenovirus. Bars indicate mean ± SEM. (C) Dispersed human islets from normal donors (n = 3–5) were transduced with control adenovirus (CTL) or with cdk6 and cyclin D3 (K6 + D3), or cdk1 and cyclin A (K1 + A), or cdk1 and cyclin E (K1 + E), or cdk2 and cyclin A (K2 + A), or cdk2 and cyclin E (K2 + E), without (top panels) or with (bottom panels) Ad.p27kip1. Immunolabelling for Ki67 is shown in red, insulin in white and DAPI in blue. (D) Quantification of data in C at 72 hours after transduction. Bars indicate mean ± SEM of the % of the proliferation in each condition without Ad.p27kip1.
