Figure 6 | Scientific Reports

Figure 6

From: In vitro and in vivo study of epigallocatechin-3-gallate-induced apoptosis in aerobic glycolytic hepatocellular carcinoma cells involving inhibition of phosphofructokinase activity

Figure 6

F2,6BP could not reverse the effect of EGCG on PFK siRNA knockdown HCC cell glycolysis and apoptosis.

(A) Western blot analysis was used to detect the transduction efficiency of PFK in HCC cells. Actin served as a loading control. (B–E) PFK siRNA knockdown HCC-LM3 and HepG2 cells were treated with EGCG (100 μM) with or without F2,6BP (10 μM) for 24 h. At the time points indicated, the following measurements were performed: cell proliferation rate (B), lactate production (C), 2-DG uptake (D), western blot analysis of cleaved-caspase 3, cleaved-caspase 8, cleaved-caspase 9 and β-actin (E). Plotted values represent the mean ± standard error of three independent experiments (n = 3) (+P < 0.01).

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