Table 2 Analytical parameters for the individual substrate, metabolite and internal standard (IS).
Analyte | Ionization mode | Precursor ion (m/z) | Product ion (m/z) | Fragmentor (V) | Collision energy (eV) |
|---|---|---|---|---|---|
Phenacetin | ESI+ | 180.1 | 110.2 | 110 | 21 |
4-acetamidophenol | ESI+ | 152.1 | 110.1 | 90 | 15 |
Bupropion | ESI+ | 240.1 | 184.1 | 90 | 8 |
Hydroxybupropion | ESI+ | 256.1 | 238.1 | 70 | 8 |
Tolbutamide | ESI− | 269.2 | 170.0 | 100 | 12 |
4-hydroxytolbutamide | ESI− | 285.2 | 186.0 | 100 | 12 |
Dextromethorphan | ESI+ | 272.3 | 147.1 | 150 | 33 |
Dextrorphan | ESI+ | 258.1 | 157.1 | 150 | 42 |
Chlorzoxazone | ESI− | 168.0 | 132.0 | 110 | 20 |
6-hydroxychlorzoxazone | ESI− | 184.1 | 120.0 | 90 | 17 |
Midazolam | ESI+ | 326.1 | 291.0 | 170 | 28 |
1-hydroxymidazolam | ESI+ | 342.1 | 324.0 | 130 | 20 |
3-acetamidophenol (IS) | ESI+ | 152.1 | 93.0 | 80 | 26 |
Chlorpropamide (IS) | ESI− | 275.1 | 190 | 100 | 11 |
Mebendazole (IS) | ESI+ | 296.1 | 264.1 | 150 | 22 |
