Table 5 List of TaqMan probes and primers of the 6 storage Tribolium species.
Secies | Primers and Probes | Sequence 5′-3′ | bp | Tm(°C) | Product (bp) |
|---|---|---|---|---|---|
T.destructor | TdeF | CGTACAGAACTAGGAAAC | 18 | 58.1 | 116 |
TdeR | CCGATTATTATAGGTATTACTATG | 24 | 57.8 | ||
TdeP | FAM-TCCTTAATCGGGAATGACCAAAT-BHQ | 23 | 65 | ||
T. brevicornis | TbrF | GAGCAGTAGCAATTACAG | 18 | 58.9 | 84 |
TbrR | TTCGGTCGGTTAATAATATAG | 21 | 58.7 | ||
TbrP | FAM-TCACTTCCAGTGTTAGCCGGTG-BHQ | 22 | 69.6 | ||
T. madens | TmaF | TCCTGGTTCTCTAATTGG | 18 | 59.3 | 138 |
TmaR | GCTCCTAGTATAAGTGGAA | 19 | 59.1 | ||
TmaP | FAM-AATGTAATTGTCACAGCCCATGC-BHQ | 23 | 67.1 | ||
T. freemani | TfrF | CGTAGATTTAGCAATTTTCAGG | 22 | 61.7 | 169 |
TfrR | TGAAAGAAGTAGAAGAATAGCG | 22 | 61.9 | ||
TfrP | FAM-AGCTGGTATCTCATCAATTCTTGGAGC-BHQ | 27 | 69.8 | ||
T. castaneum | TcaF | GATCCTCTGTTGATCTTG | 18 | 58.1 | 183 |
TcaR | CAGGAAGAGATAAGAGAAG | 19 | 57.5 | ||
TcaP | FAM-TCTGGGAGCAGTTAATTTCATTACAAC-BHQ | 27 | 66.8 | ||
T. confusum | TcoF | CAGGATGAACTGTTTACC | 18 | 58.7 | 151 |
TcoR | GTAGGTCGTATATTAATTACTG | 22 | 57.3 | ||
ToP | FAM-ATCATCTAATATCGCTCACGGAGGAG-BHQ | 26 | 68.6 |
