Figure 4: C/EBPβ mRNA expression in different tissues and EMSA analysis of C/EBPβ binding to the BPI promoter. | Scientific Reports

Figure 4: C/EBPβ mRNA expression in different tissues and EMSA analysis of C/EBPβ binding to the BPI promoter.

From: Identification of a 5-Methylcytosine Site that may Regulate C/EBPβ Binding and Determine Tissue-Specific Expression of the BPI Gene in Piglets

Figure 4

(a) C/EBPβ mRNA expression in different tissues, C/EBPβ was only highly expressed in the lungs. (b) Nuclear proteins from duodenal tissues expressing C/EBPβ were extracted using the nuclear protein extraction kit. After addition of biotin-labeled probe (BPI wt, lane 1 to 5; BPI mut, lane 7 to 9), C/EBPβ antibody (lane 5) and incubation at RT for 30 min, DNA-protein complexes were analyzed by EMSA with 5% polyacrylamide gel.

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