Figure 1
From: R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5
R-spondin 2 (Rspo2) is highly expressed in laser capture microdissection-harvested spinal motor neurons (SMNs) of the mouse spinal cord.
(A) Toluidine blue-stained section of the cervical spinal cord of a 6-week-old C57BL6/J mouse before laser capture microdissection. Arrows indicate SMNs to be dissected. (B) The left anterior horn region (enlarged from A) after the dissection of SMNs. Orange lines mark the traces of the laser beam. (C) The right posterior horn region (enlarged from A) after the dissection of posterior horn cells. Orange line marks the trace of the laser beam. (D) A representative dissected SMN. (E) The ratio of mRNA expressions in SMNs and posterior horn cells of Agrn, Chat, Isl1, Mnx1 and Wnt-related genes (Rspo, Wnt, Fzd and Lrp genes) according to the Affymetrix microarray data. Agrn, Chat, Isl1, Mnx1, Fzd and Lrp encode agrin, choline acetyltransferase, islet-1, HB9, frizzled and low-density lipoprotein receptor-related protein, respectively. (F) In situ hybridization of Rspo2 in the cervical spinal cord of a 6-week-old C57BL6/J mouse.
