Table 1 Endothelial cells are the main targets of DEET.

From: The insect repellent N,N-diethyl-m-toluamide (DEET) induces angiogenesis via allosteric modulation of the M3 muscarinic receptor in endothelial cells

Proliferation (% of control ± SEM)

DEET 10−8 M

DEET 10−5 M

pFHHSiD 10−7 M DEET 10−8 M

pFHHSiD 10−7 M DEET 10−5 M

U87MG

98.21 ± 1.59

97.18 ± 1.28

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HUVEC

113.55 ± 3.16*

112.03 ± 2.90*

102.79 ± 5.41

100.82 ± 5.23

  1. CyQUANT assay reveals that both concentrations of DEET (10−8 M and 10−5 M) do not increase proliferation of U87MG cell line while increasing HUVEC one after 24 h treatment. The selective M3 muscarinic receptor antagonist pFHHSiD prevents the DEET-induced endothelial proliferation. Results are expressed at mean ± SEM; *p < 0.05 compared to control (Kruskal-Wallis with Dunn’s multiple comparison test).
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