Figure 3: NhoI endonuclease activity assays. | Scientific Reports

Figure 3: NhoI endonuclease activity assays.

From: Expression and purification of the modification-dependent restriction enzyme BisI and its homologous enzymes

Figure 3

(A) NhoI digestion of phage XP12 (all m5C), pBRFM (ScaI-linearized, three m5C in Gm5CNGm5CNGC), and T4gt DNA (hm5C). Enzyme dilution factors are indicated on the top of each lane. (B) A theoretical digest of pBRFM (NEBcutter)25 by ScaI and another enzyme cleaving GCNGCNGC (expected sizes in bp: 1168, 966, 828, 515/504/484, 333/297, 153).

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