Figure 2
From: Highly pathogenic avian influenza H5N1 virus delays apoptotic responses via activation of STAT3
Apoptosis and necrosis in HBECs and AECs after influenza infection.
Representative pictures of apoptotic cells (in green) and nucleus (in blue) are shown in (A) HBECs at 30 hpi and (B) AECs at 24 hpi with magnification of 400×. (C) Percentage of apoptotic cells at indicated time points upon mock and influenza virus infection in HBECs and AECs are shown. Percentages of apoptosis was derived from the number of TUNEL staining positive cells over the number of DAPI stained nuclei. Results are means ± SEM of at least three biological replicates, *p < 0.05, **p < 0.01, ***p < 0.001 calculated by two way ANOVA with Bonferroni’s post-test. (D) LDH level in the culture supernatants of cells with mock or influenza infection were measured in HBECs and AECs. Results are means ± SEM of at least three biological replicates, *p < 0.05 compared to corresponding mock and calculated by two way ANOVA with Bonferroni’s post-test.
