Figure 4: Western blot analysis of ERK1/2 activation in BLA, NAc, Hippo, and Cau in controls and CP/CPPS rats.

(A) Representative Western blots. (B) Quantification of ERK1/2 phosphorylation level (pERK1/2) in BLA, NAc, Hippo, and Cau in controls (n = 8) and CP/CPPS rats (n = 8) using densitometric analysis of the bands shown in (A). Expression levels values normalized to β-actin levels. *Indicate significance levels of p < 0.05 vs. controls. The data are presented as means ± SD.