Figure 1
Disruption of XBP1 gene in SMCs decreased angiogenesis in ischemic tissue.
The right femoral arteries were banded at two adjacent sites with internal part cut to totally block foot blood supply in XBP1loxP/loxP(WT, n = 7) or SM22-Cre+/XBP1loxP/loxP(XBP1smcko, n = 21) mice. The blood flow (A) at both sides was measured by Doppler Laser Scanner at 30 minutes, 7 days and 14 days post-surgery. After blood flow measurement, the mice were humanely sacrificed and the skeletal muscle tissues around the banding site were harvested and subjected to cryo-sectioning and double immunostaining with anti-CD31 (red) and anti-SMA (green) antibodies (B). (A) Left panel shows the representative images from one mouse of each group at 14 days post-surgery. The white open box indicates the blood flow data collecting area. The right panel shows the average blood flow perfusion ratio of the right to the left foot from all mice in each group. (mean ± SEM, *P < 0.05; ANOVA, Dunnett post-test). (B) Left panel shows the representative images of blood vessels in the skeletal muscle tissues from one mouse of each group at 14 days post-surgery. Nucleus was conterstained with DAPI. Scale bar: 100 μm. Right panel shows the average vessel density (all vessels including capillary vessels) in the tissues collected from all mice in each group. (mean ± SEM, *P < 0.05; Student T-test).
