Figure 2 | Scientific Reports

Figure 2

From: XBP1 splicing triggers miR-150 transfer from smooth muscle cells to endothelial cells via extracellular vesicles

Figure 2

XBP1 splicing in SMCs increased EC migration via secreted factors.

HSMCs were infected with Ad-null (null), Ad-XBP1s (1s), or Ad-XBP1u (1u) at 10 MOI for 24 hr. The medium was then replaced by M199 medium supplemented with 0.5% FBS and incubated for 8 hr. The conditioned medium was then applied to HUVECs for proliferation assays via cell number counting (A) 24 hr post incubation) and cell migration assessments via wound healing assays (B) 6 hr post incubation, scale bar: 200 μm) and transwell migration assays (C) 6 hr post incubation, scale bar: 25 μm). Data presented are representative images or mean ± SEM of three independent experiments. (*P < 0.05; ANOVA, Dunnett post-test).

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