Figure 7: EA stimulation promotes CREB activation in the corpus callosum following prolonged cerebral hypoperfusion.

(A,B) Double immunofluorescent staining of pCREB and NG2 or CNPase in the sham control condition and histogram analysis of pCREB-positive cells also identified as OPCs and OLs. Arrowheads indicate double-labeled cells. Scale bar = 25 μm. (C,D) Numbers of pCREB-positive (pCREB+) cells at days 14 and 28 post-BCAS induction and pCREB histogram analysis. Scale bar = 100 μm. (E) pCREB Western blot images. The histogram indicates the mean (±SEM) of 3 independent experiments. Data expressed as the mean (±SEM). *P < 0.05, and ***P < 0.001 vs. sham control; #P < 0.05, or ###P < 0.001 vs. BCAS group.