Figure 3
The FAK/PI3K/Akt pathway is mediated by leptin-induced VEGF-C expression.
(A) JJ012 cells were transfected with oligonucleotide against OBRl for 24 h then incubated with leptin for 24 h. The VEGF-C mRNA level was measured by qPCR. JJ012 cells were pretreated with indicated pharmacological inhibitors or pretransfected with indicated siRNAs then incubated with leptin for 24 h. The VEGF-C mRNA level and protein expression in culture medium were measured by qPCR (B,C) and ELISA (D,E). JJ012 cells treated with leptin for the indicated time were applied to western blotting with antibodies against FAK, PI3K and Akt pathway (F). JJ012 cells pretreated with indicated pharmacological inhibitors then incubated with leptin for 15 min were applied to western blotting with antibodies against PI3K (G) and Akt (H) pathway. The quantitative results were expressed as mean ± SEM. *P < 0.05 as compared with control group; #P < 0.05 as compared with leptin-treated group.
