Figure 2: HAS2 inhibition antagonizes REAC-mediated action on Bmi1 gene expression.
From: REAC technology and hyaluron synthase 2, an interesting network to slow down stem cell senescence
At each indicated passage, ADhMSCs were left untreated (control) or treated for 12 hours with REAC, with or without 1 mM 4-MU. The mRNA level of Bmi1 was normalized to HPRT1 and was expressed as fold change relative to mRNA level at time 0 (untreated cells at passage 5), defined as 1. At each time point, mRNA levels from REAC-treated ADhMSCs were significantly different from those detected in control untreated cells. mRNA levels from cells that had been treated with REAC in the presence of 4-MU were significantly different from the expression levels detected in REAC-treated cells in the absence of the HAS2 inhibitor. (mean ± S.E.; n = 6; P < 0.05).
