Figure 3: HAS2 inhibition blunts the effect of REAC treatment on the transcription of stemness related genes.
From: REAC technology and hyaluron synthase 2, an interesting network to slow down stem cell senescence
At each indicated passage, ADhMSCs were left untreated (control) or subjected to REAC treatment for 12 hours, in the absence or presence of 1 mM 4-MU. The mRNA levels of Oct4 (A), Sox2 (B), or Nanog (C) were normalized to HPRT1 and were expressed as fold of change relative to mRNA level at time 0 (unexposed cells at passage 5), defined as 1. At each time point, mRNA levels from REAC-treated ADhMSCs were significantly different from those detected in control untreated cells. mRNA levels from cells that had been treated with REAC in the presence of 4-MU were significantly different from the expression levels detected in REAC-treated cells in the absence of the HAS2 inhibitor. (mean ± S.E.; n = 6; P < 0.05).
