Table 1 Altered gene expression in Δndk compared to PAO1.
Gene locus | Name | Gene function | log2 ratio (Δndk/WT) | P vaule |
|---|---|---|---|---|
T3SS | ||||
PA1690 | pscU | translocation protein in type III secretion | 3.02 | 1.05E-20 |
PA1691 | pscT | translocation protein in type III secretion | 4.84 | 1.06E-23 |
PA1692 | PA1692 | translocation protein in type III secretion | 4.37 | 1.11E-26 |
PA1693 | pscR | type III secretion system protein | 1.23 | 1.30E-18 |
PA1694 | pscQ | type III secretion system protein | 6.87 | 6.27E-106 |
PA1695 | pscP | translocation protein in type III secretion | 17.11 | 2.30E-204 |
PA1696 | pscO | translocation protein in type III secretion | 16.91 | 3.66E-77 |
PA1697 | PA1697 | type III secretion system ATPase | 8.15 | 0 |
PA1698 | popN | type III secretion outer membrane protein PopN | 4.80 | 8.74E-160 |
PA1699 | pcr1 | hypothetical protein | 17.30 | 5.92E-59 |
PA1700 | pcr2 | hypothetical protein | 7.06 | 3.27E-41 |
PA1701 | pcr3 | hypothetical protein | 16.28 | 2.10E-38 |
PA1702 | pcr4 | hypothetical protein | 4.21 | 5.90E-19 |
PA1703 | pcrD | type III secretory apparatus protein PcrD | 2.02 | 9.36E-80 |
PA1704 | pcrR | transcriptional regulator PcrR | 1.91 | 3.68E-06 |
PA1705 | pcrG | type III secretion regulator | 5.24 | 1.90E-83 |
PA1706 | pcrV | type III secretion protein PcrV | 5.64 | 0 |
PA1707 | pcrH | regulatory protein PcrH | 6.31 | 1.94E-209 |
PA1708 | popB | translocator protein PopB | 5.50 | 0 |
PA1709 | popD | translocator outer membrane protein PopD | 5.99 | 0 |
PA1710 | exsC | exoenzyme S synthesis protein C | 4.05 | 0 |
PA1711 | exsE | hypothetical protein | 4.12 | 5.97E-235 |
PA1712 | exsB | exoenzyme S synthesis protein B | 4.42 | 2.33E-175 |
PA1713 | exsA | transcriptional regulator ExsA | 4.06 | 4.80E-157 |
PA1714 | exsD | hypothetical protein | 4.78 | 0 |
PA1715 | pscB | type III export apparatus protein | 5.84 | 2.10E-115 |
PA1716 | pscC | type III secretion outer membrane protein PscC | 3.51 | 4.36E-184 |
PA1717 | pscD | type III export protein PscD | 6.02 | 7.01E-76 |
PA1718 | pscE | type III export protein PscE | 5.21 | 3.45E-31 |
PA1719 | pscF | type III export protein PscF | 3.92 | 7.80E-50 |
PA1720 | pscG | type III export protein PscG | 4.01 | 3.03E-46 |
PA1721 | pscH | type III export protein PscH | 3.95 | 2.41E-33 |
PA1722 | pscI | type III export protein PscI | 4.28 | 3.92E-39 |
PA1723 | pscJ | type III export protein PscJ | 3.06 | 2.39E-47 |
PA1724 | pscK | type III export protein PscK | 3.94 | 9.94E-12 |
PA1725 | pscL | type III secretion system protein | 2.49 | 2.20E-16 |
PA3841 | exoS | exoenzyme S | 3.69 | 0 |
PA0044 | exoT | exoenzyme T | 4.54 | 0 |
PA2191 | exoY | adenylate cyclase | 2.97 | 2.05E-46 |
PA3842 | spcS | chaperone | 3.69 | 1.27E-68 |
QS signal molecule synthetase and regulator | ||||
PA1432 | lasI | autoinducer synthesis protein LasI | −3.12 | 0 |
PA3476 | rhlI | autoinducer synthesis protein RhlI | −2.09 | 0 |
PA0996 | pqsA | coenzyme A ligase | −5.81 | 0 |
PA0997 | pqsB | alkyl quinolones biosynthesis protein | −6.96 | 0 |
PA0998 | pqsC | alkyl quinolones biosynthesis protein | −5.91 | 4.61E-260 |
PA0999 | pqsD | 3-oxoacyl-synthase III | −4.22 | 0 |
PA1000 | pqsE | Quinolone signal response protein | −4.12 | 8.01E-254 |
PA2587 | pqsH | FAD-dependent monooxygenase | −4.45 | 0 |
PA1430 | lasR | transcriptional regulator LasR | −1.03 | 1.42E-240 |
PA3477 | rhlR | transcriptional regulator RhlR | −3.39 | 0 |
PA1003 | mvfR | transcriptional regulator MvfR (PqsR) | −1.31 | 6.81E-64 |
PA1898 | qscR | quorum-sensing control repressor | 3.32 | 7.68E-65 |
Virulence associated exoproducts | ||||
PA3724 | lasB | elastase LasB | −6.76 | 0 |
PA1871 | lasA | LasA protease | −7.03 | 0 |
PA1246 | aprD | alkaline protease secretion protein AprD | −2.92 | 2.19E-116 |
PA1247 | aprE | alkaline protease secretion protein AprE | −2.95 | 5.20E-102 |
PA1248 | aprF | alkaline protease secretion outer membrane protein AprF | −2.50 | 3.21E-55 |
PA1249 | aprA | alkaline metalloproteinase | −1.25 | 0 |
PA0026 | plcB | phospholipase C | −2.43 | 1.02E-219 |
PA4210 | phzA1 | phenazine biosynthesis protein | −0.98 | 0.051979 |
PA4211 | phzB1 | phenazine biosynthesis protein | −2.21 | 1.12E-53 |
PA4212 | phzC1 | phenazine biosynthesis protein PhzC | −1.81 | 3.68E-06 |
PA4214 | phzE1 | phenazine biosynthesis protein PhzE; | −2.42 | 4.68E-08 |
PA4217 | phzS | flavin-containing monooxygenase | −2.05 | 8.77E-114 |
PA1899 | phzA2 | phenazine biosynthesis protein | −3.04 | 1.79E-22 |
PA1900 | phzB2 | phenazine biosynthesis protein | −4.89 | 0 |
PA1901 | phzC2 | phenazine biosynthesis protein PhzC | −4.26 | 1.81E-58 |
PA1130 | rhlC | rhamnosyltransferase | −3.18 | 0 |
PA3478 | rhlB | rhamnosyltransferase subunit B | −7.00 | 0 |
PA3479 | rhlA | rhamnosyltransferase subunit A | −6.69 | 0 |
PA2570 | lecA | PA-I galactophilic lectin | −5.82 | 0 |
