Figure 1 | Scientific Reports

Figure 1

From: Bivalent vaccine platform based on Japanese encephalitis virus (JEV) elicits neutralizing antibodies against JEV and hepatitis C virus

Figure 1

Identification of the positions for foreign epitope insertion in JEV E protein.

(A) Schematic representation of plasmid used for expression of JEV prM and E. Amino acid sequence of the JEV E protein is shown. The positions for insertion of FLAG-tag or HCV E2 neutralization epitope are indicated by arrows. CAG: CAG promoter, dC: deleted partial C-terminal 22 aa of capsid. (B) Detection of JEV E protein in cell lysates and supernatants of 293T cells transfected with each plasmid. Detection was performed at 2 days post-transfection by immunoblotting using anti-JEV mouse serum or anti-FLAG antibody. (C) Detection of JEV E protein in cell lysates and supernatants of 293T cells transfected with each plasmid. Detection was performed at 2 days post-transfection by immunoblotting using anti-JEV mouse serum. For source data, see Supplementary Figure 1B,C.

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