Figure 6

Neutralizing antibodies against JEV and HCV in sera of mice immunized with SVP-E1.

(A) The binding of monoclonal antibody E1-#384 or normal mouse IgG to JEV SVPs was determined by FCS analysis. (B) JEV was pre-incubated with 5000-fold diluted sera for 1 h and then used to infect Vero cells. Cultures were incubated for 48 h and infection then was enumerated by counting E-positive foci using immunofluorescence. Infectivity is expressed as the percentage of virus infection without serum. (C) HCVtcp derived from genotype-1b, -2a, or -3a viruses were preincubated for 1 h with 100-fold diluted sera from mice immunized with saline or SVP-E1 and then used to infect Huh7.5.1 cells. Luciferase activity was determined at 48 h post-infection and is expressed relative to activity without serum. The statistical significance of differences between groups was evaluated using the Student’s t-test (*P < 0.01, **P < 0.001 vs. serum from saline-immunized control group).