Table 2 Primer sequences of target and reference genes.
From: Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration
Gene | Gene name | Accession number | Primer sequence 5′ → 3′ |
|---|---|---|---|
Col 2 | Collagen type 2, alpha 1 | NM_001844.4 NM_033150.2 | TTTCCCAGGTCAAGATGGTC CTGCAGCACCTGTCTCACCA |
Col 1 | Collagen type 1, alpha 1 | NM_000088.3 | CGGCTCCTGCTCCTCTTAG CACACGTCTCGGTCATGGTA |
ACAN | Aggrecan | NM_013227.3 NM_001135.3 | TGAGGAGGGCTGGAACAAGTACC GGAGGTGGTAATTGCAGGGAACA |
OCN | Osteocalcin; Bone gamma-carboxyglutamate (gla) protein | NM_199173.4 | ATGAGAGCCCTCACACTCCTC GCCGTAGAAGCGCCGATAGGC |
Col 9 | Collagen, Type IX, alpha 1 | NM_001851.4 | GTGTTGCTGGTGAAAAGGGT GGGATCCCACTGGTCCTAAT |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM_001289745.1 NM_002046.5 NM_001289746.1 NM_001256799.2 | GGGCTGCTTTTAACTCTGGT TGGCAGGTTTTTCTAGACGG |
