Figure 8
AMPK siRNA inhibited the expression of its downstream proteins.
BMSCs were transfected with siRNA targeting AMPK or nonspecific scramble siRNA (control siRNA) for 48 h and then treated with APN (30 μg/mL) for 18 h, followed by exposure to FSS (15 dynes/cm2) for 24 h. Cell lysates were collected as described in the Materials and Methods section and subjected to Western blotting analysis with antibodies specific to p-AMPK, AMPK, p-ACC and ACC; AMPK and ACC served as controls. The statistics are representative of bands corresponding to the control and treatment groups. The results are expressed as the mean ± SD, n = 6. aaP < 0.05 vs. the control siRNA + 15 dynes/cm2 FSS-treated group; bbP < 0.05 vs. the control siRNA+ 30 μg/mL APN + 15 dynes/cm2 FSS-treated group; ccP < 0.05 vs. the AMPK siRNA+ 30 μg/mL APN + 15 dynes/cm2 FSS-treated group.
