Figure 9
APN protected against FSS-induced changes in cellular viability and cytokines in rat BMSCs via AMPK.
BMSCs were transfected with siRNA targeting AMPK or nonspecific scramble siRNA (control siRNA) for 48 h and then treated with APN (30 μg/mL) for 18 h, followed by exposure to FSS (15 dynes/cm2) for 24 h. The cellular viability and cytokine levels were assessed as described previously. (A) Cell viability and representative morphology of rat BMSCs. (B) Levels of bFGF. (C) Levels of TGF-β. (D) Levels of VEGF. (E) Levels of PDGF. The results are expressed as the mean ± SD, n = 6. aaP < 0.05 vs. the control siRNA + 15 dynes/cm2 FSS-treated group; bbP < 0.05 vs. the control siRNA + 30 μg/mL APN + 15 dynes/cm2 FSS-treated group; ccP < 0.05 vs. the AMPK siRNA + 30 μg/mL APN + 15 dynes/cm2 FSS-treated group.
