Figure 3: C12-HSL induces mitochondrial dysfunction in LS174T cells.

(A) Flow cytometric analysis of mitochondrial membrane potential in LS174T cells treated with AHLs. (B,C) Flow cytometric analysis of mitochondrial ROS and (D) western blot showing protein expression of active-caspase3. Values are presented as mean ± SEM (n = 6) in (C) Mitosox Fluorescence and (D) protein content. *p < 0.05, ***p < 0.001 versus DMSO group.