Figure 1

Representative micrographs of (a) unprocessed control cells, and cells dried in 300 mM trehalose to (b) 1.11 gH2O/gdw, (c) 0.27 gH2O/gdw, and (d) 0.01 gH2O/gdw. All cells were stained with Hoeschst 33342 (blue) and cells with a compromised membrane were stained with Sytox (green). Micrograph bar represents 50 microns.