Figure 1
Palladin associates with focal adhesions and modulates focal adhesion maturation.
(A) TIRF images of EGFP-palladin cell spreading on glass showing palladin organization as it changes from a diffuse localization into mature adhesions and stress fibers. Scale bar: 15 μm. (B) Left panel: Dual color image of a cell expressing EGFP-palladin (green) and mCherry-paxillin (red) showing focal adhesions and stress fibers. Scale bar: 5 μm. Right panel: Kymograph along the direction of growth of a focal adhesion (as indicated by white line in the left), showing accumulation of paxillin and palladin in a focal adhesion. Scale bars: 3 μm horizontal, 2 min vertical. TIRF image of (C) EGFP-palladin cell and (D) Palladin knockdown (Palld4) cell transfected with mApple-paxillin (red) showing multiple focal adhesions along the cell periphery. Scale bar: 15 μm. Plot of the mean fluorescence intensity of a focal adhesion as a function of time during adhesion maturation in (E) an EGFP-palladin cell and (F) a palladin knockdown cell. (G) Beeswarm graphs showing comparison of the maturation times of focal adhesions in EGFP-palladin and Palld4 cells (p < 0.0001, Wilcoxon ranksum test). (H) Comparison of focal adhesion length in EGFP-palladin and Palld4 cells (p < 0.01, Wilcoxon rank sum test). The box shows the interquartile range of the data, spanning from first quartile to the third quartile of the data, red line indicates the median and whiskers denote 1.5x interquartile range.
