Figure 2 | Scientific Reports

Figure 2

From: The actin crosslinking protein palladin modulates force generation and mechanosensitivity of tumor associated fibroblasts

Figure 2

Palladin knockdown impairs radial fiber formation.

(A) Snapshot of EGFP-palladin cell labeled with Rhodamine-phalloidin for actin showing strong radial fibers (RSF). A typical RSF is indicated by the arrow. Scale bar: 15 μm. (B) Snapshot of EGFP-palladin cell showing localization of palladin in RSF. Scale bar: 15 μm. (C) Merged image showing co-localization of actin and palladin in RSF. (D) Zoomed in image of the region the dotted boxed from (C) showing RSF in greater detail. (E) Snapshot of palladin KD cells (Palld4) labeled with Rhodamine-phalloidin showing a lack of radial stress fibers in the cell. Scale bar: 15 μm. (F) Snapshot of a control shRNA cell (PGIPZ) showing the presence of radial fibers as indicated by the arrow for an example. Scale bar: 15 μm for all panels. (G) Bar graph showing comparison of the percentage of cells (EGFP-palladin, control sh-RNA (PGIPZ) and palladin KD (Palld4)) which displayed radial fibers quantified at 4 hours after spreading initiation (N = 30 for each condition).

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