Figure 5 | Scientific Reports

Figure 5

From: The actin crosslinking protein palladin modulates force generation and mechanosensitivity of tumor associated fibroblasts

Figure 5

Palladin and myosin localize in alternate bands across actin stress fibers.

(A) Widefield fluorescence image of EGFP-palladin cell labeled with mCherry-myosin-IIA showing localization of palladin (green) and myosin (red) in a spread cell. Scale bar: 15 μm. (B) Magnified image of the square highlighted in (A) showing alternating bands of palladin (green) and myosin (red) along actin stress fibers. Scale bar: 5 μm. (C) Intensity profile of the line highlighted in (B) showing alternating intensity peaks of palladin (green, lower profile) and myosin (red, upper profile) fluorescence. (D) Bar graph comparing the band spacing for palladin and myosin.

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