Figure 4
CB2 receptor activation induces autophagy in macrophages through an HO-1 dependent pathway.
(A) Left, representative images (original magnification x400) and quantification of HO-1 (red) and F4/80 (green) double positive peritoneal macrophages isolated from WT mice and exposed to 5 μM of JWH-133 or its vehicle for 6 hours. Right, HO-1 mRNA expression in peritoneal macrophages isolated from WT mice and exposed to 5 μM of JWH-133 or its vehicle for 6 hours. (B) Representative images (original magnification x400) and quantification of the number of LC3 (red)-positive dots per F4/80 (green)-positive peritoneal macrophages exposed to 2 μM of ZnPP and 5 μM of JWH-133 or vehicle for 6 hours in the presence of 10 μM of chloroquine. (C) Representative images (original magnification x400) and quantification of the number of SQSTM1/p62 (red)-positive dots per F4/80 (green)-positive peritoneal macrophages exposed to 2 μM of ZnPP and 5 μM of JWH-133 or vehicle for 6 hours in the absence of chloroquine. Data are mean ± SEM. #p < 0.05 for JWH-133 vs vehicle and & p < 0.05 for ZnPP vs vehicle.
