Figure 2: Binding of c-di-GMP to Bc2 RNA riboswitch enhances transcription read-through of cap gene. | Scientific Reports

Figure 2: Binding of c-di-GMP to Bc2 RNA riboswitch enhances transcription read-through of cap gene.

From: Cyclic di-GMP contributes to adaption and virulence of Bacillus thuringiensis through a riboswitch-regulated collagen adhesion protein

Figure 2

(a) The relative expression levels of the 5′-UTR of cap in comparison with the complete cap gene by qPCR analyses in the BMB171 strain. (b) The differential expression levels of cap in the ΔBc2 and BMB171 strain by qPCR. Expression of gapdh gene was served as a negative control. (c) Products of in vitro transcription of DNA templates coding for the wild type (WT) and substitution mutant (A11T) riboswitches. The A11T mutant carries a single A to T mutation in the c-di-GMP binding pocket. FL and T denote full length and terminated transcripts, respectively. (d) The predicted secondary structures of Bc2 RNA in the absence and presence of c-di-GMP. Nucleotides predicted to bind to c-di-GMP are highlighted in red, and nucleotides predicted to form the terminator hairpin in the absence of c-di-GMP are highlighted in olive. The coding sequence of cap is boxed. Error bars depict SD of data from three independent experiments. ***P < 0.001.

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