Figure 3: High levels of intracellular c-di-GMP promote cap expression in B. thuringiensis. | Scientific Reports

Figure 3: High levels of intracellular c-di-GMP promote cap expression in B. thuringiensis.

From: Cyclic di-GMP contributes to adaption and virulence of Bacillus thuringiensis through a riboswitch-regulated collagen adhesion protein

Figure 3

(a) Different intracellular c-di-GMP concentrations of the Δ2dgc, Δ3pde and BMB171 strains as determined by LC-MS/MS. (b) qPCR analyses of differential transcription profiles of cap in the Δ2dgc and Δ3pde mutants in comparison with the parent strain BMB171. (c) β-galactosidase activity analyses for B. thuringiensis strains carrying the Pcap-lacZ, PcapΔ-lacZ or promoterless Pnull-lacZ transcriptional fusion plasmids. (d) β-galactosidase activity analyses for B. thuringiensis strains carrying either Pcap-lacZ or Pcap-lacZ site-mutants or promoterless Pnull-lacZ transcriptional fusion plasmids. (e) Model showing proposed regulation of cap transcription by Bc2 RNA. Error bars depict SD of data from three independent experiments. ***P < 0.001; **P < 0.01; *P < 0.05.

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