Figure 4

Static images of simultaneous SPECT/CT imaging at 30 min after injection of ^99mTc-3P-RGD₂ and ¹³¹I-NGA in ^99mTc-window (red), ¹³¹I-window (green) and fusion window.

Untreated mice were used as normal controls (a,f). For fibrotic group (b,c), ^99mTc-3P-RGD₂ showed significant higher liver accumulation than that of normal mice at 30 min. Highest accumulation was found in 8 week fibrotic liver. While the accumulations of ¹³¹I-NGA in fibrotic liver much lower than that in normal liver. The ratios of Fib/Nor derived from dual-isotope imaging protocols (e) were consistent well with that of single isotope imaging (d). Liver uptake value was calculated by drawing ROIs on image and the normal liver uptake was set as 1. In the ^99mTc-window, for tumorous mice (g), activity is found in tumor and kidney. While for normal mice (f), no tumor lesion was found. In ¹³¹I-window, a surprisingly high accumulation of ¹³¹I-NGA in residual normal liver parenchyma was achieved. (h) Liver uptakes of ¹³¹I-NGA in normal and tumorous mice were calculated by drawing ROIs on single and dual-isotope SPECT images, respectively. (i) Tumor and liver uptakes of ^99mTc-3P-RGD₂ in tumor bearing mice were obtained from single and dual-isotope SPECT imaging, respectively. Data were expressed as means ± SD (n = 3). In all panels, ^*P < 0.05 versus normal mice; ^#P < 0.05 versus FIB-4 W group.