Figure 3: Liver fibroblasts express IL-34 in NAFLD patients. | Scientific Reports

Figure 3: Liver fibroblasts express IL-34 in NAFLD patients.

From: Interleukin-34 as a fibroblast-derived marker of liver fibrosis in patients with non-alcoholic fatty liver disease

Figure 3

Liver specimens were obtained from patients with non-alcoholic fatty liver disease (NAFLD) (liver cirrhosis/Stage 4 (A) and advanced fibrosis/Stage 3 (B). Serial sections from these resected liver specimens were labeled as follows: mouse anti-human interleukin-34 (IL-34) monoclonal and secondary goat anti-mouse antibody (Ab) (IL-34 staining), mouse anti-human alpha smooth muscle actin (α–SMA) Ab and secondary goat anti-mouse Ab (α-SMA staining), secondary goat anti-mouse Ab (negative control for IL-34 staining). Arrows in the (A,B) panels indicate the fibroblasts expressing IL-34. Immunofluorescence staining on frozen liver specimens from patients with NAFLD was shown (C) (x200, green, IL-34; red, α-SMA; blue, DAPI). White arrowheads mark the positions of IL-34 and α-SMA positive cells. Inset of the photomicrograph shows fluorescent merged cells in enlarged scale.

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