Figure 1: Desmoglein-3 and flotillin-2 colocalise in human epidermis and human epithelial cell lines. | Scientific Reports

Figure 1: Desmoglein-3 and flotillin-2 colocalise in human epidermis and human epithelial cell lines.

From: Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes

Figure 1

Human skin cryosections, HaCaT and MCF10A cells were immunostained for Dsg3 (red) and flotillin-2 (green). In human epidermis, flotillin-2 is expressed throughout the epidermal layer and colocalises with Dsg3 in keratinocytes of the stratum basale and suprabasale. In HaCaT keratinocytes, flotillin-2 is localised at the cell membrane and in intracellular vesicular structures. Dsg3 is localised at the plasma membrane where it colocalises with flotillin-2. In MCF10A mammary epithelial cells, flotillin-2 and Dsg3 show a more profound colocalisation at the sites of cell-cell contact. The cell lines were grown on glass coverslips for at least 3 days. Scale bar: 20 μm.

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