Figure 4: Expression of adhesion proteins in flotillin-2 knockout mouse skin.

(a) Skin of adult wildtype (WT) and flotillin-2 knockout (Flot-2-KO) mice was lysed, and the levels of adhesion proteins were analysed by Western blot. (b–e) Western blot signals for Dsg3, Dsg1, E-cadherin and γ-catenin were quantified by scanning densitometry and normalised against GAPDH. Each data point represents the relative protein amount in a mouse skin lysate. A randomly chosen wildtype sample was set as 1, and all other samples were normalised to it. The mean ± SD is indicated for each protein. Number of analysed animals: (b) WT: 21, Flot-2-KO: 15. (c) WT: 21, Flot-2-KO: 15. (d) WT: 13, Flot-2-KO: 12. e) WT: 14, Flot-2-KO: 10.