Figure 7: Exogenous EGFP gene silencing effect. | Scientific Reports

Figure 7: Exogenous EGFP gene silencing effect.

From: Polysaccharide Nanoparticles for Efficient siRNA Targeting in Cancer Cells by Supramolecular pKa Shift

Figure 7

Bright-field, fluorescence, and merged images of PC-3 cells after treatment with (a–c) Lipofectamine 2000 + EGFP-pDNA (negative control); and Lipofectamine 2000 + EGFP-pDNA followed by (d–f) EGFP-siRNA, (g–i) Lipofectamine 2000 + EGFP-siRNA, (j–l) CB[6]‒ADA‒HACD + EGFP-siRNA (experimental group), respectively. (m) Ratios and (n) geometric mean values of fluorescence intensity of fluorescent PC-3 cells according to the results of the FCM experiments: (i) negative control; and Lipofectamine 2000 + EGFP-pDNA followed by (ii) Lipofectamine 2000 + EGFP-siRNA, (iii) Lipofectamine RNAiMAX + EGFP-siRNA, (iv) X-tremeGENE + EGFP-siRNA, (v) experimental group, (vi) CB[6]‒ADA‒HACD + HA + EGFP-siRNA, (vii) CB[6]‒ADA + EGFP-siRNA, and (viii) HACD‒ADA + EGFP-siRNA, respectively.

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