Figure 4: 3,6-DHF suppresses Notch signaling pathway in BC cells. | Scientific Reports

Figure 4: 3,6-DHF suppresses Notch signaling pathway in BC cells.

From: 3,6-dihydroxyflavone suppresses the epithelial-mesenchymal transition in breast cancer cells by inhibiting the Notch signaling pathway

Figure 4

(A) Western blot detections for the level of Notch1, NICD, Hes-1 and c-Myc in MDA-MB-231 and MCF-7 cells treated with different doses of 3,6-DHF (0, 5, 10, and 20 μM) for 24 h. (B) The effect of 3,6-DHF treatment for 24 h on NICD-CSL-MAML functional unit formation in MDA-MB-231 cells detected by immunoprecipitation combined western blots. (C) Western blot detections for the level of Notch1 and NICD in breast tumors. (D) Immunofluorescence detections of Notch1 and Hes-1 levels in MDA-MB-231 cells treated with different doses of 3,6-DHF (0, 5, 10, and 20 μM) for 24 h. (E) Immunohistochemistry detections of Notch1, NICD and Hes-1 expression in breast tumors.

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