Figure 5: Notch1 over-expression reduces the inhibitory effects of 3,6-DHF on EMT and BCSCs. | Scientific Reports

Figure 5: Notch1 over-expression reduces the inhibitory effects of 3,6-DHF on EMT and BCSCs.

From: 3,6-dihydroxyflavone suppresses the epithelial-mesenchymal transition in breast cancer cells by inhibiting the Notch signaling pathway

Figure 5

(A) Effects of 3,6-DHF treatment for 24 h on the expressions of Notch1, NICD, snail, twist, slug and E-cadherin in un-transfected MDA-MB-231 cells (UTC), cells transfected with the plasmid of pcDNA3-Notch1 (TCNotch1) and cells transfected with miR-34a inhibitor (TCanti-34a). Effects of 3,6-DHF treatment for 24 h on the migration (B), invasion (C), ALDH-positive population (D) and mammosphere formation (E) in UTC, TCNotch1 and TCanti-34a cells. The data are presented as the means ± SD (n = 3). Each data point represents the mean SEM of three independent experiments. *P < 0.05, **P < 0.01 compared with control; #P < 0.05, ##P < 0.01 compared with UTC.

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