Figure 7
From: Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage
Validation of IL-24 expression.
(A) Microarray top 3 up/downregulated genes (B) Gene set enrichment analysis of differentially methylated genes and whole genome expression array. IL-24 was a top hit candidate after this analysis (C) IL-24 expression on mRNA level. Increased expression of IL-24 in MT-MET-4 cells was confirmed via Q-PCR. Error bars depict the SD of three independent experiments. P values were calculated by two-tailed, unpaired sample t-test (*p < 0.05). (D,E) IL-24 expression on protein level. Secretion of the IL-24 protein and intracellular IL-24 protein measured in supernatant and cell lysate of MET-4 and MT-MET-4, respectively. Error bars depict the SD of three independent experiments. P values were calculated by two-tailed, paired sample t-test. (*p < 0.05, **p < 0.01, ***p < 0.005).
