Figure 2 | Scientific Reports

Figure 2

From: Long Non-coding RNA H19 Inhibits Adipocyte Differentiation of Bone Marrow Mesenchymal Stem Cells through Epigenetic Modulation of Histone Deacetylases

Figure 2

H19 and miR-675 inhibited adipogenic differentiation of BMSCs.

(A) Images of Oil red O staining in BMSCs transfected with H19, H19-mut, miR-675, or their control vectors (NC, miR-NC) on day 10 of differentiation. Scale bar, 500 μm. Histograms show quantification of Oil red O staining by spectrophotometry (normalized to control groups). (B) Relative mRNA expression of the adipogenic factors PPARγ, C/EBPα and FABP4 measured by qRT-PCR on day 10 of adipogenic induction in BMSCs transfected with H19, H19-mut, miR-675, or their control vectors. (C) Western blot analysis of PPARγ, C/EBPα, FABP4 and GAPDH on day 10 of adipogenic induction in BMSCs transfected with H19, H19-mut, miR-675, or their control vectors. Histograms show quantification of the band intensities. Results are presented as mean ± SD (*P < 0.05, **P < 0.01).

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