Figure 3 | Scientific Reports

Figure 3

From: Long Non-coding RNA H19 Inhibits Adipocyte Differentiation of Bone Marrow Mesenchymal Stem Cells through Epigenetic Modulation of Histone Deacetylases

Figure 3

Knockdown of H19 and miR-675 promoted adipogenic differentiation of BMSCs.

(A) Images of Oil red O staining in BMSCs transfected with shH19, anti-miR-675, or their scrambled vectors (shNC, miR-NC) on day 10 of differentiation. Scale bar, 500 μm. Histograms show quantification of Oil red O staining by spectrophotometry (normalized to control groups). (B) Relative mRNA expression of the adipogenic genes PPARγ, C/EBPα and FABP4 on day 10 of differentiation in BMSCs transfected with shH19, anti-miR-675, or their scrambled vectors. (C) Western blot analysis (upper) and quantification (down) of protein expression of PPARγ, C/EBPα, FABP4 and GAPDH on day 10 of differentiation in BMSCs transfected with shH19, anti-miR-675, or their scrambled vectors. Data are presented as mean ± SD (*P < 0.05, **P < 0.01).

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