Figure 2
From: Deciphering and modulating G protein signalling in C. elegans using the DREADD technology
Amino acid alignment of transmembrane domains 3 and 5 of muscarinic acetylcholine receptor orthologs.
(A) Sequences of mAChR type 3 from 13 different species were retrieved from publically available databases such as NCBI/GenBank. Alignment was performed using the Clustal W alignment48. Shown is the amino acid sequence of transmembrane domain 3 and 5. Conserved residues are shaded in blue. Highlighted in red are the highly conserved tyrosine Y3.33 (Y146) and alanine A5.46 (A237) which are mutated in mammalian DREADDs. (B) Analogously, alignment of mAChR type 2 was performed. While Y3.33 is also conserved in C. elegans GAR-2, amino acid 5.46 differs from other muscarinic orthologs.
