Figure 1: Src is activated in rodent models of type 2 diabetes.
From: RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src
Min6 cells were treated with: (A) glucolipotoxicity (glucose 16.7 mM and 0.4 mM palmitate) for 24 h; (B) TNF-α (80 nM) for 6 h; or (C) LPS (10 μg/mL) for 24 h, and cell extracts were collected. (D) Isolated islets from ICR or db/db mice. Cell extracts were analysed by western blotting using antibodies against p-Src (Tyr416) and Src. β-actin was detected as an internal control. (E) Islets were isolated from ICR or db/db mice. IFA was performed with antibodies directed against p-Src (red) and insulin (green). Insulin with green staining was used to identify β cells (scale bar = 100 μm). Data are means ± SEM of three separate experiments.
