Figure 3: RIG-I is upregulated in MIN6 cells.
From: RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src
Min6 cells were stimulated with glucolipotoxicity, LPS or retinoic acid (RA) (10 μM), or transfected with a plasmid encoding RIG-I for 24 h, as indicated, and cell extracts were collected. (A) QRT-PCR to detect the mRNA levels of RIG-I in Min6 cells. (B) Cell extracts were analysed by western blotting using antibodies against RIG-I. Islets were isolated from ICR or db/db mice. (C) QRT-PCR analysis and (D) western blotting were performed to detect the mRNA or protein levels of RIG-I in primary cells. Quantification of the relative protein content of RIG-I is shown. β-actin was detected as an internal control. (E) Min6 cells were treated with glucolipotoxicity, LPS or RA or transfected with a plasmid encoding RIG-I for 24 h, as indicated. IFA was performed with antibodies against RIG-I (red); DAPI was used for nuclear staining (blue) (scale bar = 120 μm). Data are means ± SEM of three separate experiments. *P < 0.05 versus control.
