Figure 5: Elevated RIG-I inhibits proliferation via stability of p27 in pancreatic β cell line.
From: RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src
24 h after transfection of a plasmid encoding RIG-I, Min6 cells were treated with IGF-1 and RA, as indicated. (A) The mRNA levels of the indicated genes, including those encoding positive cell cycle regulators cyclin D1, cyclin E, CDK2, and negative regulators p21 and p27, were determined by qRT-PCR. Cell extracts were analysed by western blotting with antibodies against (B) positive and (C) negative cell cycle regulators. (D) MIN6 cells were pre-treated with or without MG132 (20 μM) for 1 h and subsequently treated as indicated. Cell extracts were analysed by western blotting with antibodies against P27. β-actin was detected as an internal control. Data are means ± SEM of three separate experiments. *P < 0.05 versus control.
