Figure 6: RIG-I-induced downregulation of Skp2 is involved in the stability of P27 in a pancreatic β cell line.
From: RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src
24 h after transfection of plasmid encoding RIG-I, Min6 cells were treated with IGF-1 and RA, as indicated in the above experiments. (A) The mRNA and (B) protein levels of Skp2 were determined by qRT-PCR and western blotting, respectively. Min6 cells were transfected with a plasmid encoding Skp2 for 24 h and subsequently treated with (C) RA or (D) glucolipotoxicity, as indicated. Cell extracts were analysed by western blotting with antibodies against Skp2 and P27. β-actin was detected as an internal control. Data are means ± SEM of three separate experiments. *P < 0.05 versus control.
