Figure 8: Transcriptional activity of STAT3 was suppressed by RIG-I accumulation.
From: RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src
(A) Min6 cells were transfected with si-RIG-I for 24 h, and then treated with RA. Protein levels of p-STAT3 and STAT3 were determined by western blotting. (B) 24 h after transfection of a plasmid encoding RIG-I, Min6 cells were treated with IGF-1 and RA, as indicated. Western blotting was used to detect the protein levels of p-STAT3 and STAT3. (C) Min6 cells were treated with IGF-1 or RA, as indicated. IFA was performed with an antibody against p-STAT3 (green); DAPI was used for nuclear staining (blue) (scale bar = 100 μm). (D) Luciferase activity of pGMSTAT3-Lu (a STAT3-dependent reporter) was measured and shown as the fold change. (E) Islets were isolated from ICR or db/db mice. Protein levels of p-STAT3 and STAT3 were determined by western blotting in primary islet cells. β-actin was detected as an internal control. Data are means ± SEM of three separate experiments. *P < 0.05 versus control.
