Figure 9: RIG-I interrupts the binding between Src and STAT3.
From: RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src
(A) 24 h after transfection of a plasmid encoding RIG-I, Min6 cells were treated with IGF-1 or RA, as indicated. (B) RA was used to treat Min6 cells after 24 h transfection of si-RIG-I. (C) Min6 cells were transfected with STAT3 for 24 h, and then treated with RA. All these prepared extracts were immunoprecipitated with an antibody against p-Src. Immunocomplexes or whole cell extracts (WCE) were analysed by western blotting with antibodies against RIG-I or STAT3, as indicated. (D) Min6 cells were treated as in panel (C), and DNA synthesis was analysed using EdU labelling assays. Representative micrographs of EdU labelling assays in MIN6 cells are shown (scale bar = 120 μm). The percentage of EdU-positive β cells was quantified. Data are means ± SEM of three separate experiments. *P < 0.05 versus control.
