Figure 3
From: Cargo binding promotes KDEL receptor clustering at the mammalian cell surface
KDELR/cargo clustering is dose- and temperature-dependent.
(A) Changes in cargo accumulation of eGFP-RTAHDEL at the surface of HeLa cells cultivated at 25 °C or 37 °C. The 3-fold reduced activity in simulations (left) represents the known effect of temperature on intracellular transport processes (e.g. endocytosis and exocytosis). (right) The experimental results at 25 °C and 37 °C. (B) Effect of changing the concentration of the model cargo eGFP-RTAHDEL on KDELR/cargo clustering at the plasma membrane. The indicated rates in simulations (left) were adopted to obtain the best fits to the experimental data (right).
