Figure 4
From: Cargo binding promotes KDEL receptor clustering at the mammalian cell surface
Preferential arrival sites of KDELRs at the plasma membrane.
(A) The log-log plots of cluster-size distribution P(s) of eGFP-RTAHDEL (160 μg/ml) treated HeLa cells at the indicated time points. The dashed line corresponds to the best power-law fit P(s) ~ s−β with 
. (B) Evolution of the receptor clusters at the plasma membrane. A randomly chosen region of the cell surface is shown at different time points (see Suppl. Info. for the detailed description of the methodology of distinguishing the clusters and obtaining the cluster-size distribution). (C) A comparison of the resulting P(s) from different receptor dynamic scenarios in simulations. The solid, dashed and dotted lines denote the shape of P(s) at t = 120 min for randomly distributed immobile receptors, aggregation process including lateral diffusion of receptors and nearest-neighbor attraction between them and preferential attachment process, respectively. (D) The frequency of vesicle arrival at a sample cell periphery over a time window of 500 s. (E) In vivo dynamics of mCherry-ERD2.1. The transfected HeLa cells with mCherry-ERD2.1 were analyzed by CLSM (720 frames/h). The illustrated heat map represents the accumulated fluorescent signals of successive frames. The regions with high traffic load, e.g. around Golgi, are eliminated to provide a more clear color distinction near the cell surface. (F) The frequency of vesicle transport near the plasma membrane of untreated or eGFP-RTAHDEL treated cells. The data is averaged over bins of size 10 μm (**P ≤ 0.01, t test).
